Digital Drosophila Archive
15 lines
2.0 KiB
Markdown
15 lines
2.0 KiB
Markdown
# Phase 4X — evidence and interpretation limits
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Checked 2026-09-29. No external IDs replace MaleCNS canonical IDs.
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- Author repository [funkelab/synister_malecns](https://github.com/funkelab/synister_malecns) describes MaleCNS transmitter prediction and links its ground-truth source. A prediction pipeline is not a measurement of every cell's transmitter or receptor-dependent current sign.
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- [flyconnectome/drosophila_neurotransmitters](https://github.com/flyconnectome/drosophila_neurotransmitters) is the linked author ground-truth resource. This audit does not import updated labels or infer correspondence from names alone.
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- [Haynes et al., eLife 2015](https://pmc.ncbi.nlm.nih.gov/articles/PMC4305081/), doi:10.7554/eLife.03868, reports DPM GABA/5-HT signaling and inhibitory effects in its tested preparation. This motivates a DPM interpretation warning; it does not assign a replacement sign to these exact MaleCNS bodies.
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- [Eckstein et al., Cell 2024](https://pmc.ncbi.nlm.nih.gov/articles/PMC11106717/), doi:10.1016/j.cell.2024.03.016, discusses DPM prediction discrepancies in its studied datasets. Prior 4W source-access limitations remain recorded; no claim of having retrieved the blocked full text anew.
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`predicted_nt_confidence` and `celltype_predicted_nt_confidence` retain their actual raw names and numeric values. No independently calibrated probability interpretation, confidence threshold, or physiology-based sign correction is introduced.
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Raw `ground_truth` is reported as a supplied field. Agreement of consensus with that field is not an independent test of prediction accuracy. Exact annotation class `DAN`, exact type `DPM`, transmitter metadata, and prior exact-compartment connectivity mapping are distinct evidence axes. None alone establishes appetitive/aversive function, dopamine concentration, or the current-sign response of every target.
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This fixes audit semantics, not biology. Frozen runtime semantics, including dopamine=+1, remain unchanged. Any alternative receptor/modulatory model requires a separate explicit hypothesis and validation.
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