Digital Drosophila Archive
3.1 KiB
Phase 4Z: event-resolved PN feedback and input acceptance
This diagnostic stage follows user approval to investigate upstream feedback and delivered-input differences. All historical inputs and model files remain read-only. No learning, gain adjustment, changed thresholds, novel transmitter sign, mask expansion or game interface is permitted.
Registered scope
Replay six old calibration odors with seed 131101 under the two existing Phase 4Y conditions: frozen reference and diagnostic DPM fast-current-off. This is reproduction of a sealed intervention, not promotion of it. Only that previous in-memory effective-current overlay is allowed. Save outputs in new Phase 4Z directories. Preserve current delay/refractory and request acceptance semantics. Do not force a matched delivered spike train by overriding refractory state.
Observer targets are KC 82312, every actual annotation-ALPN input to it, and ALLN nodes 1197/1381/1511 found on a prior Phase 4W time-supported ancestry. Selection is exploratory and explicitly based on prior outcomes. Do not call it blind validation or unique-cause identification.
Use exact incoming-edge event reconstruction and CPU single-cell replay to verify queue, V/g, natural spikes, output spikes and accepted requests. Conditional omissions retain other measured arrivals and APL; they are not closed-loop whole-brain ablations. Distinguish anatomical recurrence, event-supported recurrence and experimentally demonstrated necessity.
Reference reproduction must match Phase 4Y counts, final V/g, trace and KC events. Frozen-reference whole-brain events must additionally match Phase 4W. Repeat both first-odor conditions and rerun five frozen deterministic reference trials. Abort result generation on any mismatch; do not repair frozen data. Hash history and all new final artifacts.
Literature context, not parameter authority
- Eckstein et al., Cell 2024, doi:10.1016/j.cell.2024.03.016: DPM dopamine predictions in FlyWire/hemibrain identified as mispredictions. This is not direct ground truth for the two MaleCNS bodies. https://pmc.ncbi.nlm.nih.gov/articles/PMC11106717/
- Haynes et al., eLife 2015, doi:10.7554/eLife.03868: DPM GABA/serotonin-related physiology; does not provide a universal MaleCNS edge-specific current model. https://pmc.ncbi.nlm.nih.gov/articles/PMC4305081/
- Hu et al., PNAS 2010, doi:10.1073/pnas.0914912107: functional mushroom-body-to-antennal-lobe feedback. Existence of such feedback does not prove the current residual loop traverses KC/MBON. https://pubmed.ncbi.nlm.nih.gov/20479249/
- Localized inhibition in the Drosophila mushroom body, 2020: spatially localized APL inhibition. No changes to frozen APL parameters are authorized here. https://pmc.ncbi.nlm.nih.gov/articles/PMC7541083/
- Shiu et al., Nature 2024, doi:10.1038/s41586-024-07763-9: sensorimotor LIF model, not universal validation of associative-memory physiology. https://pmc.ncbi.nlm.nih.gov/articles/PMC11446845/
No slow receptor time constants, biological sign correction or parameter tuning is derived from these citations. Source code SHA-256 serves as this audit's reproducible version identifier.