Archive MaleCNS research sources, provenance, bibliography and immutable data inventory

2026-09-29 03:38:32 +08:00
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+# Phase 4W — transmitter interpretation addendum
+
+本补充独立保存，不改写已封存 Phase 4W 或之前任何产物，不修改 neurotransmitter/sign，不运行新动力学实验。
+
+**重要限定：4W 表格中的 DAN 是按 neurotransmitter=dopamine 得到的操作性分组，不是全部已经生物学确认的 DAN。**
+其中出现 annotation_type=DPM 的细胞。因此 omit_DAN 的效果不能表述为“已经证明奖励 DAN 导致持续活动”。
+
+| target neuron_idx | DPM 占全部晚期正向输入 | DPM 占 metadata-dopamine 组正向输入 |
+|---:|---:|---:|
+| 59832 | 7.0606% | 29.4592% |
+| 78836 | 31.2165% | 34.1652% |
+| 82312 | 0.0000% | 0.0000% |
+
+原始生理研究报道 DPM 的 GABA/5-HT 信号及对蘑菇体的抑制作用。[Haynes et al., eLife 2015](https://pmc.ncbi.nlm.nih.gov/articles/PMC4305081/)
+2024 EM 递质分类论文亦讨论 DPM 在其研究的 hemibrain/FlyWire 数据中被预测为 dopamine 的误预测问题；这不是对本 MaleCNS 具体 body 的实验定性。[Eckstein et al., Cell 2024](https://pmc.ncbi.nlm.nih.gov/articles/PMC11106717/)
+2024 论文直接 PMC 访问遇到 CAPTCHA、ETH PDF 遇到 403；相关正文段落来自检索索引，书目信息由 PubMed 核对。没有声称已完整读取受限全文。
+下一步应先核对原始 transmitter 字段、置信度、cell-type 身份和当前 sign 语义，明确区分递质预测、受体作用与快速 LIF 电流假设。不能直接按论文改 sign，更不能为 PASS 改符号。
+尚未做 DPM-only 或全脑闭环因果干预；本轮条件回放与输入重建仍成立，但生物学归因必须受上述限制。
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+{
+  "status": "TRANSMITTER-IDENTITY-INTERPRETATION-WARNING",
+  "DAN_label_definition_in_4W": "neurotransmitter metadata equals dopamine, not independently verified anatomical DAN identity",
+  "DPM_input_metadata": [
+    {
+      "neuron_idx": 2403,
+      "body_id": 12569,
+      "annotation_class": null,
+      "annotation_type": "DPM",
+      "annotation_subclass": null,
+      "annotation_superclass": "cb_intrinsic",
+      "neurotransmitter": "dopamine",
+      "audit_category": "DAN"
+    }
+  ],
+  "late_positive_contributions": [
+    {
+      "target_neuron_idx": 59832,
+      "DPM_fraction_all_positive": 0.0706060454249382,
+      "DPM_fraction_metadata_dopamine_positive": 0.29459211230278015,
+      "metadata_dopamine_fraction_all_positive": 0.239673912525177
+    },
+    {
+      "target_neuron_idx": 78836,
+      "DPM_fraction_all_positive": 0.3121650218963623,
+      "DPM_fraction_metadata_dopamine_positive": 0.3416522741317749,
+      "metadata_dopamine_fraction_all_positive": 0.9136922359466553
+    },
+    {
+      "target_neuron_idx": 82312,
+      "DPM_fraction_all_positive": 0.0,
+      "DPM_fraction_metadata_dopamine_positive": 0.0,
+      "metadata_dopamine_fraction_all_positive": 0.00624917633831501
+    }
+  ],
+  "frozen_signs_changed": false,
+  "simulations_rerun": false,
+  "conclusion": "Do not interpret omit_DAN as selective removal of confirmed reward DANs. It removes the operational metadata-dopamine group, including DPM.",
+  "causal_limit": "Neither DPM-only necessity nor whole-brain causal necessity was tested. Literature is not proof of the true transmitter/sign for these exact MaleCNS bodies.",
+  "source_access": {
+    "Haynes2015": "PMC full text retrieved",
+    "Eckstein2024": "PubMed bibliographic record and PMC/ETH search-index excerpts; PMC CAPTCHA and ETH direct PDF 403",
+    "MaleCNS_authors_repository": "https://github.com/funkelab/synister_malecns"
+  },
+  "sources": [
+    {
+      "title": "Haynes et al. 2015, A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster",
+      "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC4305081/",
+      "doi": "10.7554/eLife.03868"
+    },
+    {
+      "title": "Eckstein et al. 2024, Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster",
+      "url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC11106717/",
+      "doi": "10.1016/j.cell.2024.03.016"
+    }
+  ],
+  "sealed_4W_integrity_before": {
+    "root": "/data/digital-drosophila/experiments/phase4w-late-kc-feedback",
+    "checked": 214,
+    "pass": true,
+    "control_sha256": "cbc90feefc335c1b49f2121e5364459dc2f1c6d4dc6d5b3cf1a7ecb1a5058dc7"
+  },
+  "sealed_4W_integrity_after": {
+    "root": "/data/digital-drosophila/experiments/phase4w-late-kc-feedback",
+    "checked": 214,
+    "pass": true,
+    "control_sha256": "cbc90feefc335c1b49f2121e5364459dc2f1c6d4dc6d5b3cf1a7ecb1a5058dc7"
+  }
+}
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+{
+  "source_sealed_experiment": "/data/digital-drosophila/experiments/phase4w-late-kc-feedback",
+  "files": [
+    {
+      "path": "DPM-observed-metadata.parquet",
+      "sha256": "e05155107183b281ea6035d3bdb252424cc83e7a6465f262b3514c88d2c4fe4f",
+      "bytes": 5157
+    },
+    {
+      "path": "REPORT.md",
+      "sha256": "743441c4cabc7a82e048962841598453f3971335662ecbbb786fac800e5cdcc0",
+      "bytes": 1656
+    },
+    {
+      "path": "interpretation.json",
+      "sha256": "8165f4cc41587f0a3d08b40089f07fff3d9471633376c993147888302de8136b",
+      "bytes": 2913
+    },
+    {
+      "path": "phase4w_transmitter_note.py",
+      "sha256": "c971e6dd91d6d497a602f420f9929afa7aa7de7ce21867e4e63caabbdca138f1",
+      "bytes": 5377
+    }
+  ]
+}